Trying to Prevent Mycoplasma

Project Title

Use of Antimicrobial Peptides as Adjuvants for Vaccines Against Mycoplasma Bovis

Researchers

Dr. Jose Perez-Casal jose.perez-casal@usask.ca

Jose Perez-Casal, PhD, Volker Gerdts, PhD (Vaccine and Infection Disease Organization, University of Saskatchewan)

Status Project Code
Completed December, 2010 0007-106

BACKGROUND

Mycoplasma bovis has become a significant bacterial pathogen in commercial feedlots over the past decade or so. Since Mycoplasma bacteria lack a cell wall, antibiotics (which disturb bacterial cell walls) are not very effective for treating cattle infected with these bacteria. M. bovis is involved in bovine respiratory disease complex (BRD), and also plays a role in chronic pneumonia and polyarthritis syndrome (CPPS) in high-risk fall-placed feedlot calves. This syndrome has emerged as a leading cause of mortality in feedlot calves in Western Canada. Calves with CPPS may also be euthanized due to severe lameness problems. The disease is quite complex. There are many different strains of M. bovis, and although many calves become infected, not all become sick, and not all develop CPPS.  Recent research has demonstrated that antimicrobial peptides possess antibacterial and immune system boosting properties.  These peptides are part of an animal’s innate immune system and act directly upon bacteria.

OBJECTIVES

  • To determine if small chains of amino acids known as antimicrobial peptides in combination with a Mycoplasma bovis antigen can protect against mycoplasma by stimulating a stronger immune response in newborn calves.

WHAT THEY DID

The researchers examined, in detail, the methods by which M. bovis affects various immune system functions.  With that knowledge, they constructed a series of antigen-antimicrobial peptide combinations, and tested their effectiveness on M. bovis in culture.  A proof of concept trial was then performed to test the different combinations in live cattle infected with mycoplasma.  The animals were immunized twice, 21 days apart and the animals exhibiting the best immune response to the vaccine were transported to the VIDO research farm, along with the control group.  Animals were then challenged with both Bovine Herpes Virus 1 (BHV-1) and M. bovis.  They were monitored for signs of lameness, depression and respiratory distress, and body weights and temperature were monitored daily for 22 days after the challenge.  At the end of the trial, all animals were euthanized and necropsies performed.

WHAT THEY LEARNED

The researchers discovered that mycoplasma acts on healthy cells in a number of ways.  These include inhibiting apoptosis (programmed cell death) of peripheral-blood mononuclear cells (white blood cells that play a critical role in immune responses), prolonging the life of the cell, which may facilitate the spread of M. bovis throughout various tissues.  In addition, M. bovis can invade and replicate inside red blood cells without damaging or affecting the function of those cells, creating another transport mechanism by which M. bovis can spread, as well as great way for mycoplasma to “hide” from the animal’s immune system.  M. bovis also creates biofilms, which means the cells stick together on a surface.  These biofilms are another way that M. bovis evades animal immune responses.

WHAT IT MEANS

In the preliminary animal trial that tested the pilot vaccine, all treatment groups experienced weight losses, with only a few animals able to regain most of that weight by the end of the trial.  Likewise, all animals experienced rectal temperature increases, and only the surviving animals showed a return to normal temperatures.  Survival rates were not statistically different between treatment groups and the control, and similar degrees of lung lesions were observed between all groups.  M. bovis was found in lung tissue (both healthy and diseased) in all animals, and in one animal with obvious signs of arthritis.  It was also found in the blood of 9 out of the 24 animals.  Other common pathogens associated with respiratory disease such as M. haemolytica and P. haemolytica were also found in some of the lungs of the challenge animals, which may have impaired the effectiveness of the vaccine combinations administered.