Examing Antimicrobial Sprays Applied to Beef Trim

Project Code: FOS.07.10
Completed: March 2013

Project Title:

Examining the Decontamination of Beef Trim by Spraying it with Lactic Acid Solution

Researchers:

Dr. Colin Gill gillc@agr.gc.ca

Xianqin Yang, Ph.D., and Mohamed K. Youssef, Ph.D. (AAFC Lacombe)

Background: Lactic acid has antibacterial properties, and is naturally found in yogurt and sourdough bread. It is approved by Health Canada for use on beef. Commercial packing plants routinely use 2 to 5% lactic acid to spray or wash beef carcasses and cuts in order to eliminate E. coli O157:H7 and other pathogenic E. coli. Research has shown that lactic acid can reduce E. coli O157:H7 in beef that has been experimentally inoculated with artificially high numbers of bacteria. These results have not been validated in commercial situations where E. coli O157:H7 levels are much lower. The effectiveness of lactic acid solutions on generic (non-O157) E. coli has been studied in commercial practice, but whether this can also reduce low numbers of E. coli O157:H7 is uncertain because E. coli O157:H7 is thought to be more acid-resistant than generic E. coli. Consequently, the extent to which commercial packers can use lactic acid to control E. coli O157:H7 on beef is not clear. Previous studies of lactic acid treatments of beef have also given little or no consideration as to how survival of E. coli O157:H7 may be affected by the type of meat surface, the volume of lactic acid used, or the numbers of bacteria on the meat.

Objective: To determine how effectively different concentrations and volumes of lactic acid can control different levels of generic E. coli and E. coli O157:H7 contamination on muscle, fat and membrane-covered beef surfaces.

What they did: An apparatus was constructed to evenly apply controlled volumes of lactic acid to beef trim. Fat, muscle and membrane-covered surfaces were inoculated with acid-adapted E. coli O157:H7 or non-acid adapted generic E. coli at high (100,000 cells/cm2), medium (10 cells/cm2) or low levels (1 cell/10 cm2). The three different meat samples inoculated with three levels of E. coli were then sprayed with water (no lactic acid) or 2% or 5% lactic acid, at volumes of 0.5 ml/cm2 or 0.02ml/cm2. After treatment, the numbers of surviving injured and surviving uninjured bacteria were counted separately.

What they learned: Although lactic acid sprays and washes are very beneficial for reducing microbial contamination on dressed carcasses, this study showed that lactic acid sprays had limited benefit on trim.

Although a 99% reduction was attained on fat and muscle surfaces using 5% lactic acid at 0.5 ml/cm2, E. coli and E. coli O157:H7 were not completely eliminated from beef trim, even when surfaces were inoculated with low levels of bacteria. Results also varied with the type of trim. The spray eliminated more E. coli from membrane-covered surfaces than from fat or cut muscle surfaces; membranes are smooth, but fat or cut muscle surfaces have tiny cracks that some bacteria can hide in. This means that if there are very few bacteria on the surface to begin with, it will be easier for most of them to hide in the fat and muscle cracks.

Most of the E. coli that survived the lactic acid treatment were not injured, and survival was very similar for both E. coli O157:H7 and generic E. coli.  Similarities in survival between the acid-tolerant E. coli (O57:H7) and acid-susceptible E. coli (generic) suggests that the acid wasn’t reaching the bacteria. This is probably related to the cracks in the fat and muscle. The E. coli that hide in the cracks of the fat or muscle were protected from the lactic acid treatment, and acid can’t kill E. coli that it doesn’t touch.

What it means: The presence of small cracks in fat and muscle means that there is always a place for some bacteria to be protected from organic acid washes and sprays.

Lactic acid treatment can reduce E. coli numbers but cannot completely eliminate it from beef trim, even when E. coli levels are extremely low.

Treating beef trim with lactic acid or other antimicrobial solutions will probably have very limited benefits for beef safety. Trim pasteurization, irradiation, or finding other means of reducing the amount of E. coli on beef trim would likely provide a greater benefit than antimicrobial sprays applied to beef trim.

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